Specificity
RIG 334-01 reacts specifically with H-ficolin. No cross-reaction has been observed with L- or M-ficolin.
Immunogen
Recombinant human H-ficolin
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Epitope Specificity
Not determined
Unit Size
200 µL: Cat. No. RIG 334-01-021 mL: Cat. No. RIG 334-01-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region.
