Specificity
MON U-5 is specific for human uPA. No reaction with human tissue plasminogen activator (tPA) is seenwhen tested by ELISA, immunoblotting and enzyme inhibition. No reaction with any other human plasmaproteins is seen when tested by immunoblotting. MON U-5 binds single and two-chain uPA, uPA/PAI-1complex and uPA/uPAR complex (1). A reaction is seen with LMW-uPA but not with the amino terminalfragment (ATF).
Immunogen
Native human uPA
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Epitope Specificity
MON U-5 binds to the B-chain of uPA
Unit Size
200 µL: Cat. No. MON U-5-021 mL: Cat. No. MON U-5-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development. Elevated levels of uPA are associated with poor prognosis in many types of cancer. (2)
Other Info
This product is not for futher manufacture.
