Specificity
HYB 246-04 is specific for SP-D from amniotic fluid, serum and bronchioalveolar lavage. HYB 246-04 does not influence SP-D’s binding to carbohydrates, but block binding to GP-340.
Immunogen
Native human SP-D purified from late pregnancy amniotic fluid
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Epitope Specificity
Epitope is localised within the C-terminal neck CRD (carbohydrate recognition domain) of SP-D
Unit Size
200 µL: Cat. No. HYB 246-04-021 mL: Cat. No. HYB 246-04-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular "head" regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is approximately 700 ng/ml in healthy individuals. (1)
