Specificity
CSI 006-01 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I,III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with otherconnective tissue proteins (laminin, fibronectin, elastin).
Immunogen
Acid-digested pepsin soluble dog type V collagen
Positive Species Reactivity
Human, Pig, Bovine, Dog, Sheep (ovine), Kangaroo, Rabbit
Negative Species Reactivity
Mouse, Rat, Guinea Pig, Chicken
Gene ID
1289,397533, 537387, 480684, 101115457, 100327268
Epitope Specificity
Not determined
Unit Size
200 µL: Cat. No. CSI 006-01-021 mL: Cat. No. CSI 006-01-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.
