Specificity
CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with otherconnective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion tofibronectin mediated by the heparin-binding domain.
Immunogen
Bovine corneal endothelial cells
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Epitope Specificity
Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-35
Unit Size
200 µL: Cat. No. CSI 005-32-021 mL: Cat. No. CSI 005-32-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)
