Specificity
CSI 005-17 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin.
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Positive Species Reactivity
Bovine, Human, Chicken
Negative Species Reactivity
Not determined
Gene ID
280794 (2335, 396133)
Epitope Specificity
Epitope is located in the 120kD cell binding fragment
Unit Size
200 µL: Cat. No. CSI 005-17-021 mL: Cat. No. CSI 005-17-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)
