Specificity
CSI 003-23 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-23 binds nearly as well to native vitronectin as to denatured. CSI 003-23 is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.
Immunogen
Human vitronectin purified from plasma by heparin-affinity chromatography
Positive Species Reactivity
Human, Cat (feline), Dog, Goat (caprine), Bovine, Horse
Negative Species Reactivity
Not determined
Gene ID
7448, 101100765, 480621, 100861216, 507525, 100059034
Epitope Specificity
Epitope is located in the somatomedin B domain
Unit Size
200 µL: Cat. No. CSI 003-23-021 mL: Cat. No. CSI 003-23-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognizes fibronectin. (2)
