Specificity
CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is noevidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin,collagen, laminin).
Immunogen
Extracellular matrix material from cultured bovine corneal endothelial cells
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Epitope Specificity
Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.
Unit Size
200 µL: Cat. No. CSI 002-65-021 mL: Cat. No. CSI 002-65-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers. (1)
