Specificity
CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human yENaC and recognizes the C-terminal fragment.
Immunogen
Synthetic peptide seguence: VGGSIIHKAC
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Epitope Specificity
CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human ENaC and recognizes the C-terminal fragment starting from amino acid residue 182.
Unit Size
200 µL: Cat. No. CAM 035-01-021 mL: Cat. No. CAM 035-01-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit. In the y-subunit, putative cleavage sites for prostasin, kallikrein (similar site as prostasin), elastase, and plasmin exist. Prostasin is a glycosylphosphatidylinositol anchored apical serine protease expressed in the collecting duct that activates yENaC at a site close to but in the C-terminal direction to the furin cleavage with putative release of a 43-residue inhibitory tract.
