Specificity
ABS 062-33 is specific for human NGAL (neutrophil gelatinase-associated lipocalin).
Immunogen
Human recombinant NGAL coupled to carrier and adsorbed to aluminum hydroxide gel.
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Epitope Specificity
Differs from those of the HYB 211 and ABS 062-14.
Unit Size
200 µL: Cat. No. ABS 062-33-021 mL: Cat. No. ABS 062-33-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.
