Specificity
ABS 052-121 binds human ghrelin.
Immunogen
Synthetic ghrelin (14-28)
Positive Species Reactivity
Human, Rat, Mouse, Dog, Cat
Negative Species Reactivity
Not determined
Epitope Specificity
Not determined
Unit Size
200 µL: Cat. No. ABS 052-121-021 mL: Cat. No. ABS 052-121-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormoneproduced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Twoforms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active"ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHSreceptors and increases appetite. The des-acyl form does not have these effects but may have otheractions. Plasma responses of total ghrelin to food reflect those of active ghrelin.
