Specificity
ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.
Immunogen
Synthetic human Ser3-octanoyl ghrelin-28
Positive Species Reactivity
Human, Rat (5%)
Negative Species Reactivity
Not determined
Epitope Specificity
Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with ratghrelin.
Unit Size
200 µL: Cat. No. ABS 050-45-021 mL: Cat. No. ABS 050-45-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone producedby endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms areproduced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin isacylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors andincreases appetite. The des-acyl form does not have these effects but may have other actions. Plasmaresponses of total ghrelin to food may reflect those of active ghrelin.
