Specificity
ABS 002-49 is specific for C1s proenzyme and activated free C1s and C1s in complex with C1 inhibitor.
Immunogen
Human plasma C1s Proenzyme
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Epitope Specificity
Epitope differs from that of ABS 002-09.
Unit Size
200 µL: Cat. No. ABS 002-09-021 mL: Cat. No. ABS 002-09-11 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A/G purified, from culture supernatant
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.
